WP 2: Synthesis, supplying and characterisation

No	Name of the deliverable	Lead Beneficiary	SOP - Strategy - Guidance document
			NANoREG Guidance Document
D2.01	Establishment of primary MNM sample suite and web-based resource for sample order	2
D2.02	Material data sheet on primary NM suite up-linked with internet resource	14
D2.03	Experimental evaluation of OECD methods for analysis of physicochemical MNM properties	20
D2.04	Protocol for quantitative analysis of inorganic and organic MNM surface coatings	4	SOPs available in Deliverable Report SOP for determination of organic content in MNMs by thermogravimetric analysis (TGA) SOP for assessment of the contents of water, organic compounds, and degradable inorganics in MNMs using a drying oven and a laboratory furnace SOP for determination of inorganic content in MNMs by Wavelength dispersive X-ray fluorescence (WDXRF) SOP for characterization of non-covalent bound organic surface coating on MNM by extraction and mass spectrometry SOP for determination of inorganic coating in MNMs by Transmission Electron Microscopy SOP for determination of oxygen containing functional groups on CNT by decarbonylisation, decarboxylation, and dehydration in inert atmosphere using TGA-MS
D2.05	Protocol for characterization and categorization of MNM in powders and liquid dispersions	4
D2.06	Protocols for exposure-fate characterization in ecotoxicity and in vitro studies	31	SOPs available in Deliverable Report Chapter 3: The validated probe sonicator calibration protocol for the NANOGENOTOX batch dispersion protocol Chapter 4: The validated Standard Dispersion Protocol for ecotoxicological studies Chapter 5: The validated Enhanced Dispersion Protocol (NOM-water) for ecotoxicological studies Chapter 6: The validated generic NANOGENOTOX dispersion protocol for in vitro studies
D2.07	Characteristics of test aerosols using different generation methods for inhalation stu dies	21
D2.08	Protocols for exposure-fate characterization in ecotoxicity and in vitro studies	22
		UNamur	NANoREG D2.08 SOP 01 Methodology for sedimented dose determination with CLS and PIXE techniques
		NRCWE	NANoREG D2.08 SOP 02 For measurement of hydrodynamic Size-Distribution and Dispersion Stability by DLS
		UNamur	NANoREG D2.08 SOP 03 For particle size determination of a given MNM by the CLS technique
		NRCWE	NANoREG D2.08 SOP 04 To quantify the concentration of a given MNM in cell culture media with the PIXE technique
		LEITAT	NANoREG D2.08 SOP 05 For test item preparation and comparability of results during in vitro testing
		IIT	NANoREG D2.08 SOP 06 For characterizing MNM fate in biological media and digestive fluids by multi-technique based method
		NRCWE	NANoREG D2.08 SOP 07 For determination of LDH, IL-6, IL-8 adsorption onto MNM
		NRCWE	NANoREG D2.08 SOP 08 For hydrochemical reactivity and biodurability testing using an Atmosphere-Temperature-pH-controlled SBR
		NRCWE	NANoREG D2.08 SOP 09 For SDR analyses of MNM hydrochemical reactivity and dissolution in in vitro medium
		NRCWE	NANoREG D2.08 TG Document for Environmental Exposure Characterisation
D2.09	Revised OECD methods for determination of physicochemical NM properties	40
		40	NANoREG D2.09 SOP 01 Protocol for true density measurements
		40	NANoREG D2.09 SOP 02 Protocol for effective density measurements
		20	NANoREG D2.09 SOP 03 Protocol for the measurement of water solubility
		40	NANoREG D2.09 SOP 04 Protocol for the determination of the Dispersibility
		40	NANoREG D2.09 SOP 05 Protocol for IEP determination
D2.10	Protocol for size-distribution analysis of primary NM particles in air powders and liquids	26
		26	NANoREG D2.10 SOP 01 Preparation of EM-grids containing a representative sample of a dispersed NM
		26	NANoREG D2.10 SOP 02 Transmission electron microscopic imaging of nanomaterials
		26	NANoREG D2.10 SOP 03 Qualitative description of a NM based on TEM micrographs
		26	NANoREG D2.10 SOP 04 Electron microscopic image analysis of nanomaterials
		26	NANoREG D2.10 SOP 05 Electron microscopic image analysis of primary particles in aggregated nanomaterials
D2.11	Protocol(s) for VSSA analysis of primary NM in air, powders, and liquids	61	SOPs available in Deliverable Report
D2.12	Framework and procedures for characterization and reporting of MNMs for regulatory use

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